Hypothalamic transcriptome analysis of congenic-derived F2 mice (chromosome 17:3–45 Mb) exhibiting preferential carbohydrate (versus fat) intake

ata format Raw (.csfasta) and processed (.txt) xperimental factors 2 genotypes and food intake in kilocalorie (kcal) from the carbohydrate-rich diet in a diet choice paradigm (see below). xperimental features For 2 d, mice were provided a choice between two diet mixtures containing either 78% fat/22% protein or 78% carbohydrate/22% protein (per cent by energy) and then were euthanatized for collection of whole hypothalamus. Before the 2 d test, animals completed a 10 d macronutrient diet selection test, followed by a two week wash-out on standard chow. Experimental animals used for tissue collection were chosen from a larger group based on self-selected intake of carbohydrate + protein kcal in the 10 d diet choice test: congenic cast/cast F2s from the upper quartile (n = 12) and congenic b6/b6 F2 s from the lowest quartile (n = 12). onsent N/A mple source location Baton Rouge, LA USA